Proshina L G
Aviakosm Ekolog Med. 1995;29(3):31-5.
The morphological aspects of rearrangement of macrophages in white rats' subcutaneous areolar tissue during dehydration and their impact on the recovery of osmotic homeostasis were studied. A response of macrophages to dehydration with preliminary injection of antioxidant dibunol was revealed. Macrophages respond to dehydration by a rise in number, elevated cytoplasmic activity of lipolytic enzymes (beta-oxibutyrate dehydrogenase), and a peculiar ultrastructural rearrangement, i.e. a decrease of vacuoles, their sizes, an increase of the lipid volumetric fraction. However, the higher activity of macrophages in terms of production of metabolic water is concurrent to increased malonic dialdehyde production and inhibited activity of the cell antioxidant defense (superoxide dismutase). To correct the observed changes, dehydration was performed on the background of antioxidant dibunol injection. The antioxidant and subsequent dehydration did not annul the response of macrophages to increased intrabody osmolality although slightly masked the effect of dehydration. Survivability in this group of animals was by 22% higher than in the water-deprived group. Hence, the areolar macrophages can be considered the controllers of adaptive reactions of the body during dehydration, whereas antioxidants are the correction factor for these reactions.
研究了脱水过程中大白鼠皮下疏松结缔组织内巨噬细胞重排的形态学特征及其对渗透稳态恢复的影响。揭示了巨噬细胞对预先注射抗氧化剂二丁基羟基甲苯的脱水反应。巨噬细胞对脱水的反应表现为数量增加、脂解酶（β-羟丁酸脱氢酶）的细胞质活性升高以及特殊的超微结构重排，即液泡数量减少、大小减小，脂质体积分数增加。然而，巨噬细胞在代谢水产生方面的较高活性与丙二醛产量增加和细胞抗氧化防御（超氧化物歧化酶）活性受抑制同时发生。为了纠正观察到的变化，在注射抗氧化剂二丁基羟基甲苯的背景下进行脱水。抗氧化剂及随后的脱水并未消除巨噬细胞对体内渗透压升高的反应，尽管略微掩盖了脱水的影响。该组动物的存活率比缺水组高22%。因此，疏松结缔组织巨噬细胞可被视为机体脱水过程中适应性反应的控制器，而抗氧化剂是这些反应的校正因子。
