Xue C, Kaye P
Department of Parasitology, Henan Medical University, Zhengzhou.
Zhonghua Yi Xue Za Zhi. 1995 Jun;75(6):338-40, 382.
The effects of activated macrophages on Leishmania donovani amastigotes and nitrite (NO2-) release were determined by in vitro inducement of macrophage activation with recombinant IFN-r, TNF-alpha, and rIL-3. The results indicated that rIFN-r or rTNF-alpha at 40,000U/L was able to induce a significant but modest level of macrophage activity, and synergistic effect of rIFN-r and rTNF-alpha was apparent in the induction of macrophage activation. 48 hours after macrophages were induced with both rIFN-r and rTNF-alpha, the percentage of the macrophages infected by Leishmania donovani was 16%, and the mean number of the parasite per infected macrophage was 1.2 +/- 0.04. There was a strikingly significant difference in comparison with the control group, in which the infection rate of macrophages was 62% and the mean number of the parasite per infected macrophage was 6.8 +/- 0.10. However, no significant difference was noted between rIL-3 at 1/100 concentration, alone or combined with rIFN-r, and the control in leishmanicidal activity of macrophages. An increase of NO2- in the culture supernatants was paralleled by the ability of various cytokine-activated macrophages to kill the intracellular parasites.
通过用重组干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）和重组白细胞介素-3（rIL-3）体外诱导巨噬细胞活化，来确定活化巨噬细胞对杜氏利什曼原虫无鞭毛体和亚硝酸盐（NO2-）释放的影响。结果表明，40000U/L的rIFN-γ或rTNF-α能够诱导显著但适度水平的巨噬细胞活性，并且rIFN-γ和rTNF-α在诱导巨噬细胞活化方面具有明显的协同作用。在用rIFN-γ和rTNF-α诱导巨噬细胞48小时后，被杜氏利什曼原虫感染的巨噬细胞百分比为16%，每个被感染巨噬细胞内的寄生虫平均数为1.2±0.04。与对照组相比有显著差异，对照组巨噬细胞感染率为62%，每个被感染巨噬细胞内的寄生虫平均数为6.8±0.10。然而，1/100浓度的rIL-3单独或与rIFN-γ联合使用时，在巨噬细胞杀利什曼原虫活性方面与对照组相比无显著差异。培养上清液中NO2-的增加与各种细胞因子活化的巨噬细胞杀死细胞内寄生虫的能力平行。
