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[应用巢式聚合酶链反应检测克山病患者石蜡包埋心肌组织中的肠道病毒RNA]

[Detection of enteroviral RNA in paraffin-embedded myocardial tissue from patients with Keshan by nested PCR].

作者信息

Li Y, Yang Y, Chen H

机构信息

Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Shanghai Medical University.

出版信息

Zhonghua Yi Xue Za Zhi. 1995 Jun;75(6):344-5, 382.

PMID:7553145
Abstract

Enteroviral RNA in paraffin-embedded myocardial specimens from 21 patients with Keshan disease and 21 patients with cerebral trauma was investigated by nested polymerase chain reaction. Of the 21 patients with Keshan Disease, 18 (85.7%) showed positive findings. Their myocardial tissues were infected by enterovirus previously. The results suggest that enterovirus infection is the major cause of Keshan disease in the Chuxiong district, Yunnan province.

摘要

采用巢式聚合酶链反应对21例克山病患者及21例脑外伤患者石蜡包埋心肌标本中的肠道病毒RNA进行了检测。21例克山病患者中,18例(85.7%)检测结果呈阳性。他们的心肌组织曾感染过肠道病毒。结果提示,肠道病毒感染是云南省楚雄地区克山病的主要病因。

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