Bunyard B A, Nicholson M S, Royse D J
Department of Plant Pathology, Pennsylvania State University, University Park 16802, USA.
Exp Mycol. 1995 Sep;19(3):223-33. doi: 10.1006/emyc.1995.1027.
The large subunit (28S) of the ribosomal DNA repeat of Morchella, Verpa, and Disciotis and a closely related genus (Gyromitra) was enzymatically amplified via the polymerase chain reaction. Restriction fragment length polymorphisms were found among the lines investigated and used to infer phylogenetic relationships. More variability was observed toward the 5' end than toward the 3' end of the 28S rRNA gene. The RFLP data were used to assemble a phylogenetic tree for the taxonomic group. Based on the RFLP data three black Morchella species isolates differed by approximately 0.5, 1.0, and 1.5%, respectively, from all other isolates in the Morchellaceae examined in this study. Gyromitra gigas, used as an outgroup, had approximately 6.2% difference from all members of the Morchellaceae. In some cases more genetic variation was observed intraspecifically than between putative species. Additionally, the hypothesis that Morchella is composed of only a few (possibly three) polymorphic species was supported by our findings.
通过聚合酶链反应对羊肚菌属、钟菌属、盘菌属以及一个近缘属（鹿花菌属）的核糖体DNA重复序列的大亚基（28S）进行酶促扩增。在所研究的品系中发现了限制性片段长度多态性，并用于推断系统发育关系。在28S rRNA基因的5'端比3'端观察到更多的变异性。RFLP数据用于构建该分类群的系统发育树。基于RFLP数据，三种黑色羊肚菌物种分离株与本研究中检测的羊肚菌科的所有其他分离株分别存在约0.5%、1.0%和1.5%的差异。用作外类群的大鹿花菌与羊肚菌科的所有成员有大约6.2%的差异。在某些情况下，种内观察到的遗传变异比假定物种之间的更多。此外，我们的研究结果支持了羊肚菌仅由少数（可能三种）多态性物种组成的假设。
