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波希米亚钟菌和圆锥钟菌的染色体水平基因组组装。

Chromosome-level genome assemblies of Verpa bohemica and Verpa conica.

作者信息

Yan Zhuyue, Shi Xiaofei, Cai Yingli, Sun Wenhua, He Peixin, Wu Liyuan, Zhang Jin, Guo Xing, Wang Bo, Yu Fuqiang, Liu Wei

机构信息

The Germplasm Bank of Wild Species & Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.

Key Laboratory of Chemistry in Ethnic Medicinal Resources, School of Ethnic Medicine, Yunnan Minzu University Kunming, Kunming, 650500, China.

出版信息

Sci Data. 2025 May 27;12(1):880. doi: 10.1038/s41597-025-05224-0.

Abstract

Verpa, commonly known as "early morel" or "false morel", plays an important ecological role and offers considerable economic and medicinal potential. Despite their significance, research on Verpa species, particularly V. bohemica and V. conica, remains limited. In this study, we assembled high-quality sub-chromosomal genomes of six Verpa strains using Nanopore and Illumina sequencing, with average sizes of 44.38 Mb for V. bohemica and 45.40 Mb for V. conica. Specifically, the assemblies of V. bohemica strain 21108 and V. conica strain 21120 were anchored to 26 and 25 chromosomes with Hi-C technologies, respectively. The consensus quality value (QV) of both V. bohemica and V. conica exceeded 40. In addition, an average of 11,024 and 11,052 protein-coding genes were identified for V. bohemica and V. conica, respectively, with BUSCO completeness scores ranging from 98.71% to 99.24%. Overall, these reported genomes will provide valuable genomic resources for the evolution and ecological roles research of Verpa.

摘要

钟形羊肚菌,通常被称为“早羊肚菌”或“假羊肚菌”,具有重要的生态作用,并具有相当大的经济和药用潜力。尽管它们很重要,但对钟形羊肚菌属物种,特别是波希米亚钟形羊肚菌和圆锥钟形羊肚菌的研究仍然有限。在本研究中,我们使用纳米孔测序和Illumina测序组装了六个钟形羊肚菌菌株的高质量亚染色体基因组,波希米亚钟形羊肚菌的平均大小为44.38 Mb,圆锥钟形羊肚菌的平均大小为45.40 Mb。具体来说,波希米亚钟形羊肚菌菌株21108和圆锥钟形羊肚菌菌株21120的组装分别通过Hi-C技术锚定到26条和25条染色体上。波希米亚钟形羊肚菌和圆锥钟形羊肚菌的一致质量值(QV)均超过40。此外,波希米亚钟形羊肚菌和圆锥钟形羊肚菌分别平均鉴定出11,024个和11,052个蛋白质编码基因,BUSCO完整性得分在98.71%至99.24%之间。总体而言,这些报道的基因组将为钟形羊肚菌的进化和生态作用研究提供有价值的基因组资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5857/12117090/8999f0ffadb4/41597_2025_5224_Fig1_HTML.jpg

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