肠炎沙门氏菌分离株培养滤液对三种哺乳动物细胞系的产毒性
Toxigenicity of culture filtrates of Salmonella enteritidis isolates on three mammalian cell lines.
作者信息
Hariharan H, Heaney S B, Singer J T
机构信息
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
出版信息
New Microbiol. 1995 Jul;18(3):261-6.
Culture filtrates of 28 Salmonella enteritidis isolates were tested for toxicity on Vero-, CHO-, and human foreskin fibroblast (HFF) cells. Cytopathic effects on HFF cells were extensive, and were observed even with some filtrates diluted 1:256. Vero cells showed effects with filtrates diluted up to 1:16, and CHO cells gave weak or no reaction. All isolates produced iron-binding siderophores as determined by reactions on chrome-azurol-S medium.
对28株肠炎沙门氏菌分离株的培养滤液进行了对非洲绿猴肾细胞（Vero细胞）、中国仓鼠卵巢细胞（CHO细胞）和人包皮成纤维细胞（HFF细胞）的毒性测试。对HFF细胞的细胞病变效应广泛，即使是一些稀释至1:256的滤液也能观察到这种效应。Vero细胞在滤液稀释至1:16时出现效应，而CHO细胞反应微弱或无反应。通过在铬天青S培养基上的反应确定，所有分离株均产生铁结合铁载体。
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