Saheki S, Hitsumoto Y, Bando S, Murase M, Takeuchi N, Uchida K
Department of Laboratory and Clinical Medicine, Ehime University Medical School, Osaka, Japan.
Clin Chim Acta. 1995 Jun 30;238(1):43-57. doi: 10.1016/0009-8981(95)06075-o.
In some hyperlipidemic patients, low density lipoprotein (LDL) shows several peaks (polydisperse) on polyacrylamide gel disc electrophoreses, though LDL usually shows a single peak (monodisperse). In order to clarify the relationship between the LDL polydispersion and VLDL heterogeneity, LDL and VLDL were prepared from hyperlipidemic patients sera with mono- and polydisperse LDL by sequential ultracentrifugation and fractionated by gradient ultracentrifugation and their compositions were analyzed. Polydisperse LDL was rich in triacylglycerol (TG) and poor in esterified cholesterol (CE) as compared with monodisperse LDL and consisted of the lowest and the medium density subfractions when the LDL was separated into six subfractions. The monodisperse LDL was composed of a single major subfraction of a medium density. VLDL from the patients with polydisperse LDL was relatively rich in the dense and poor in the buoyant subfractions as compared with that from the patients with monodisperse LDL. The subfractions in the former contained more CE and less TG than the corresponding subfractions in the latter. There were no significant differences in the apolipoprotein compositions between those VLDLs. The results suggest that polydisperse LDL might be originated from VLDL that differs in particle sizes, densities and compositions from ordinary VLDL.
在一些高脂血症患者中，低密度脂蛋白（LDL）在聚丙烯酰胺凝胶圆盘电泳上显示出几个峰（多分散性），而LDL通常显示为单个峰（单分散性）。为了阐明LDL多分散性与极低密度脂蛋白（VLDL）异质性之间的关系，通过连续超速离心从具有单分散和多分散LDL的高脂血症患者血清中制备LDL和VLDL，并通过梯度超速离心进行分级分离，然后分析它们的组成。与单分散LDL相比，多分散LDL富含三酰甘油（TG）而酯化胆固醇（CE）含量低，当将LDL分离为六个亚组分时，多分散LDL由最低密度和中等密度亚组分组成。单分散LDL由单一的中等密度主要亚组分组成。与来自单分散LDL患者的VLDL相比，来自多分散LDL患者的VLDL在密度较高的亚组分中相对丰富，而在浮力较大的亚组分中含量较低。前者的亚组分比后者相应的亚组分含有更多的CE和更少的TG。这些VLDL之间的载脂蛋白组成没有显著差异。结果表明，多分散LDL可能起源于颗粒大小、密度和组成与普通VLDL不同的VLDL。
