Kholodova Y D, Harris W S
University of Kansas Medical Center, Kansas City, USA.
Ukr Biokhim Zh (1978). 1995 Nov-Dec;67(6):60-5.
Density gradient ultracentrifugation and high resolution polyacrylamide gel electrophoresis (Lipoprint LDL-system) were used to examine the discrete LDL subfraction patterns in normal and hyperlipidemic patients. Two new programs for LDL and VLDL subfractions were elaborated and a new method of evaluation for cholesterol content in LDL-subfractions, based on area distribution ratio of densitograms without density gradient ultracentrifugation, was created. The predominance of cholesterol and triglycerides in dense LDL3-subclass (d-1,048-1,063 g/ml) under hypercholesterolemia and hypertriglyceridemia was found. Lipoprint LDL-electrophoresis method was developed for the characteristic of lipemic state of organism and detection and quantitation of plasma LDL subfractions.
采用密度梯度超速离心法和高分辨率聚丙烯酰胺凝胶电泳法（Lipoprint LDL系统）检测正常人和高脂血症患者的离散低密度脂蛋白（LDL）亚组分模式。精心制定了两种针对LDL和极低密度脂蛋白（VLDL）亚组分的新方案，并创建了一种基于无密度梯度超速离心的密度图面积分布比来评估LDL亚组分中胆固醇含量的新方法。发现在高胆固醇血症和高甘油三酯血症情况下，致密LDL3亚类（密度为1.048 - 1.063 g/ml）中胆固醇和甘油三酯占优势。开发了Lipoprint LDL电泳法用于评估机体的脂血状态以及检测和定量血浆LDL亚组分。
