评估用于检测猪血清中抗猪痢疾蛇形螺旋体抗体的不同血清学检测方法。

Evaluation of different serological tests for detection of antibodies against Serpulina hyodysenteriae in pig sera.

作者信息

Diarra A T, Achacha M, Mittal K R

机构信息

Groupe de Recherche sur les maladies infectieuses du porc (GREMIP), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.

出版信息

Comp Immunol Microbiol Infect Dis. 1995 Jun;18(3):215-21. doi: 10.1016/0147-9571(95)00002-p.

DOI:10.1016/0147-9571(95)00002-p

PMID:7554822

Abstract

Swine dysentery is a mucohemorrhagic diarrheal disease caused by S. hyodysenteriae. The detection of asymptomatic carriers in herds is possible by serological tests. However, cross-reactions between S. hyodysenteriae and S. innocens pose a major problem in serological diagnosis. Several serological tests were evaluated for detection of antibodies to S. hyodysenteriae such as: indirect hemagglutination, passive hemolysis, conglutination and microagglutination tests. Among the tests used, only the microagglutination test was able to detect antibodies to S. hyodysenteriae. 70 to 95% of the pigs were invariably seropositive in a single dilution of 1:10 in actively infected herds whereas the number of seropositives did not exceed 10% in presumably non-infected herds. The test was found to be simple, and reliable to be used with confidence for detection of herd infection using boiled cell suspension as an antigen.

摘要

猪痢疾是一种由猪痢疾短螺旋体引起的黏液出血性腹泻疾病。通过血清学检测可以在猪群中检测出无症状携带者。然而，猪痢疾短螺旋体和无害短螺旋体之间的交叉反应在血清学诊断中构成了一个主要问题。对几种用于检测猪痢疾短螺旋体抗体的血清学检测方法进行了评估，如间接血凝试验、被动溶血试验、胶固试验和微量凝集试验。在所使用的检测方法中，只有微量凝集试验能够检测出猪痢疾短螺旋体抗体。在活跃感染的猪群中，70%至95%的猪在1:10的单一稀释度下总是呈血清阳性，而在推测未感染的猪群中，血清阳性的数量不超过10%。该检测方法被发现简单且可靠，可以放心地使用煮沸的细胞悬液作为抗原检测猪群感染情况。