Lüning C, Wroblewski J, Obrink B, Hammarström L, Rozell B
Dept. Cell Mol. Biol., Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.
Connect Tissue Res. 1995;32(1-4):201-7. doi: 10.3109/03008209509013724.
The distribution of the cell adhesion molecule C-CAM was analyzed during tooth development, eruption and formation of the junctional epithelium in rat molars by immunohistochemistry and in situ hybridization. C-CAM could not be detected during odontogenesis until the late bell stage; then only the mRNA was demonstrated in the odontoblasts and ameloblasts. Prior to eruption, a local increase in C-CAM (mRNA and protein) was observed in the reduced enamel epithelium. During eruption, high C-CAM levels were seen in the fusion zone between the oral epithelium and the reduced enamel epithelium. In the adult rat, C-CAM remained strongly expressed in the junctional epithelium. Our study indicates that C-CAM may play a role in odontogenesis and during formation of the epithelial structures involved in tooth eruption and formation of the junctional epithelium.
通过免疫组织化学和原位杂交技术，对大鼠磨牙牙齿发育、萌出及结合上皮形成过程中细胞黏附分子C-CAM的分布进行了分析。在牙发生过程中，直到钟状期末期才能检测到C-CAM；此时仅在成牙本质细胞和成釉细胞中显示出mRNA。在萌出前，在釉质发育不全上皮中观察到C-CAM（mRNA和蛋白质）局部增加。在萌出过程中，在口腔上皮与釉质发育不全上皮之间的融合区可见高C-CAM水平。在成年大鼠中，C-CAM在结合上皮中仍强烈表达。我们的研究表明，C-CAM可能在牙发生以及参与牙齿萌出和结合上皮形成的上皮结构形成过程中发挥作用。
