Ohshima H, Sato O, Kawahara I, Maeda T, Takano Y
Second Department of Oral Anatomy, Niigata University School of Dentistry, Japan.
Connect Tissue Res. 1995;32(1-4):303-11. doi: 10.3109/03008209509013738.
Responses of immunocompetent cells, especially class II major histocompatibility complex (MHC) antigen-expressing cells, were investigated after cavity preparation in the erupted upper first molar teeth of rats, by immunohistochemistry using OX6-monoclonal antibody. In control teeth, OX6-immunopositive cells were predominantly located beneath the odontoblast layer in the dental pulp. Cavity preparation caused an acute edematous reaction between the injured odontoblasts and predentin, and most of OX6-immunopositive cells in the affected site shifted away from the pulp-dentin border. After 12-24 hours, many OX6-immunopositive cells accumulated along the pulp-dentin border and extended their cytoplasmic processes into the exposed dentinal tubules. After 72 hours, newly differentiated odontoblasts replaced the degenerated odontoblasts, and few OX6-immunopositive cells remained along the pulp-dentin border. Our data suggest that some of the class II MHC antigen-expressing cells in the dental pulp participate in the initial defense reaction and presumably serve as a biological sensor for the external stimuli arriving through the exposed dentinal tubules.
通过使用OX6单克隆抗体的免疫组织化学方法，研究了大鼠上颌第一磨牙萌出后制备洞形后免疫活性细胞，尤其是表达II类主要组织相容性复合体（MHC）抗原的细胞的反应。在对照牙齿中，OX6免疫阳性细胞主要位于牙髓成牙本质细胞层下方。制备洞形导致受损的成牙本质细胞和前期牙本质之间出现急性水肿反应，并且受影响部位的大多数OX6免疫阳性细胞从牙髓-牙本质界移位。12-24小时后，许多OX6免疫阳性细胞沿着牙髓-牙本质界聚集，并将其细胞质突起延伸到暴露的牙本质小管中。72小时后，新分化的成牙本质细胞取代了退化的成牙本质细胞，并且沿着牙髓-牙本质界几乎没有OX6免疫阳性细胞残留。我们的数据表明，牙髓中一些表达II类MHC抗原的细胞参与了初始防御反应，并且可能作为通过暴露的牙本质小管到达的外部刺激的生物传感器。
