Netten P M, Wollersheim H, Gielen M J, Den Arend J A, Lutterman J A, Thien T
Department of Medicine, University Hospital Nijmegen, The Netherlands.
Eur J Clin Invest. 1995 Jul;25(7):515-22. doi: 10.1111/j.1365-2362.1995.tb01738.x.
Microvascular research is seriously hampered by the great temporal and spatial variability of the measured skin blood flow and variation in sympathetic vasomotor reflexes within and between persons. Therefore skin vasomotor reflexes were studied before and after ulnar nerve blockade within the same person, resulting in a temporal complete denervation of the fifth finger and partial denervation of the fourth finger. Skin temperature and laser Doppler flux (LDF) were registrated to measure predominantly arteriovenous shuntflow. Measurements were performed on the palmar tip of the second and fifth finger in nine healthy volunteers, at baseline, and during a sympathetic reflex test (i.e. inspiratory gasp) and postural response test. Beat-to-beat digital blood pressure was recorded from the third and fourth finger by a Finapres device. Baseline capillary blood cell velocity (CBV) was measured at the nailfold of the second and the fifth finger. After ulnar blockade baseline skin temperature, LDF and CBV increased significantly, with respectively (mean +/- SE) 3.2 +/- 0.9 degrees C, 20.9 +/- 5.9 relative perfusion units and 0.79 +/- 0.40 mm-1 s. The percentage LDF decrease of the fifth finger during inspiratory gasp was 48.2 +/- 5.3% before and 3.1 +/- 0.9% after blockade. The postural response test showed a decrease in LDF of the fifth finger with no significant difference before and after blockade, respectively 12.3 +/- 14.7% and 8.0 +/- 2.7%, while no difference was found in the increase in digital blood pressure in the denervated fourth finger compared to both the same finger before blockade and to the third non-blocked finger.(ABSTRACT TRUNCATED AT 250 WORDS)
微血管研究受到所测皮肤血流巨大的时空变异性以及个体内部和个体之间交感血管运动反射变化的严重阻碍。因此，在同一个体内研究了尺神经阻滞前后的皮肤血管运动反射，导致第五指出现暂时完全去神经支配，第四指出现部分去神经支配。记录皮肤温度和激光多普勒血流（LDF）以主要测量动静脉分流。在9名健康志愿者的第二和第五指掌侧指尖进行测量，测量时间点包括基线、交感反射试验（即吸气喘息）期间和姿势反应试验期间。通过Finapres设备记录第三和第四指的逐搏数字血压。在第二和第五指的甲襞处测量基线毛细血管血细胞速度（CBV）。尺神经阻滞后，基线皮肤温度、LDF和CBV显著升高，分别为（平均值±标准误）3.2±0.9℃、20.9±5.9相对灌注单位和0.79±0.40mm⁻¹s。吸气喘息期间，第五指的LDF下降百分比在阻滞前为48.2±5.3%，阻滞后为3.1±0.9%。姿势反应试验显示，第五指的LDF下降，阻滞前后无显著差异，分别为12.3±14.7%和8.0±2.7%，而去神经支配的第四指的数字血压升高与阻滞前同一手指以及未阻滞的第三指相比均无差异。（摘要截短于250字）
