Weirich G F, Kochansky J P, Masler E P, Lusby W R, Feldlaufer M F, Raina A K, Svoboda J A
Insect Neurobiology and Hormone Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705-2350, USA.
Experientia. 1995 Sep 29;51(9-10):961-6. doi: 10.1007/BF01921748.
The tritium-labeled bis-norleucine analog of Helicoverpa zea pheromone biosynthesis-activating neuropeptide ([3H]NLPBAN) was incubated in vitro with hemolymph from Manduca sexta or H. zea adult females. The incubations resulted in the formation of several tritium-labeled degradation products. At a [3H]NLPBAN concentration of 0.9 microM the degradation proceeded at a very slow but physiologically plausible rate (2-10 fmol/min/microliters hemolymph). The primary [3H]NLPBAN degradation reaction in M. sexta hemolymph was not inhibited by 20 microM leupeptin, 0.1 mM amastatin, 1 mM EDTA, 1 mM EGTA, 1 mM 1,10-phenanthroline, or 2 mM 4-(2-aminoethyl)benzenesulfonyl fluoride; but secondary reactions may have been affected, as some of the inhibitors changed the radio-HPLC profile of the degradation products. It is concluded that hemolymph of M. sexta and H. zea contains peptidase(s) capable of inactivating circulating PBAN.
将玉米螟性信息素生物合成激活神经肽（[3H]NLPBAN）的氚标记双去甲亮氨酸类似物与烟草天蛾或玉米螟成年雌性的血淋巴在体外进行孵育。孵育过程中形成了几种氚标记的降解产物。在[3H]NLPBAN浓度为0.9微摩尔时，降解以非常缓慢但生理上合理的速率进行（2 - 10飞摩尔/分钟/微升血淋巴）。烟草天蛾血淋巴中[3H]NLPBAN的主要降解反应不受20微摩尔亮抑酶肽、0.1毫摩尔氨肽酶抑制剂、1毫摩尔乙二胺四乙酸、1毫摩尔乙二醇双四乙酸、1毫摩尔1,10 - 菲啰啉或2毫摩尔4 - （2 - 氨乙基）苯磺酰氟的抑制；但二级反应可能受到了影响，因为一些抑制剂改变了降解产物的放射性高效液相色谱图谱。得出的结论是，烟草天蛾和玉米螟的血淋巴中含有能够使循环中的PBAN失活的肽酶。
