Ma P W, Knipple D C, Roelofs W L
Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva 14456.
Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6506-10. doi: 10.1073/pnas.91.14.6506.
Sex pheromone biosynthesis in a number of moth species is induced by a conserved 33-amino acid amidated neuropeptide PBAN (pheromone biosynthesis-activating neuropeptide). We have isolated and characterized the Helicoverpa zea PBAN cDNA corresponding to a 766-nucleotide mRNA that is expressed in the subesophageal ganglion of adult moths. This mRNA is encoded on a transcription unit comprising 6 exons. The longest open reading frame of the cDNA encodes a 194-amino acid precursor protein that contains the PBAN peptide sequence. Proteolytic processing of this protein, which has structural features consistent with its being a preprohormone, is predicted to generate Hez-PBAN and four additional neuropeptides having a common C-terminal pentapeptide motif, Phe-Xaa-Pro-(Arg or Lys)-Leu (Xaa = Gly, Ser, or Thr), which is also found in insect pyrokinin and myotropin peptide families.
许多蛾类物种的性信息素生物合成是由一种保守的33个氨基酸的酰胺化神经肽PBAN（性信息素生物合成激活神经肽）诱导的。我们已经分离并鉴定了棉铃虫PBAN cDNA，其对应于一个766个核苷酸的mRNA，该mRNA在成年蛾的咽下神经节中表达。这个mRNA由一个包含6个外显子的转录单元编码。该cDNA最长的开放阅读框编码一个194个氨基酸的前体蛋白，其中包含PBAN肽序列。该蛋白具有与前激素原一致的结构特征，预计其蛋白水解加工会产生棉铃虫PBAN和另外四种具有共同C末端五肽基序Phe-Xaa-Pro-（Arg或Lys）-Leu（Xaa = Gly、Ser或Thr）的神经肽，这种基序也存在于昆虫速激肽和促肌肽肽家族中。
