Park M, Wolfner M F
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853-2703, USA.
Dev Biol. 1995 Oct;171(2):694-702. doi: 10.1006/dbio.1995.1315.
Acp26Aa is a Drosophila seminal fluid protein that plays a role in the elevation of egg-laying by the mated female and has structural features of a prohormone. The protein, which has a region of sequence similarity to the egg-laying hormone of Aplysia, is transferred to the Drosophila female during mating. Acp26Aa is processed in the mated female's genital tract. We show here that the processing involves sequential proteolytic cleavages, and we map the position of these cleavages. Although Acp26Aa is not cleaved in the male, its processing in the mated female requires activities donated by the male. Acp26Aa ectopically expressed in unmated females is not processed. Processing of Acp26Aa in wild-type females mated to males with altered seminal fluid is dependent on the presence and amount of male accessory gland secretions. The need for molecular cooperation between the sexes for processing of Acp26Aa could restrict its activity to the mated female.
Acp26Aa是一种果蝇精液蛋白，在提高交配后雌蝇的产卵量方面发挥作用，并且具有前激素的结构特征。该蛋白与海兔产卵激素存在序列相似区域，在交配过程中会转移到果蝇雌蝇体内。Acp26Aa在交配雌蝇的生殖道中进行加工处理。我们在此表明，这种加工涉及顺序性蛋白水解切割，并确定了这些切割的位置。尽管Acp26Aa在雄蝇体内不会被切割，但其在交配雌蝇体内的加工需要雄蝇提供的活性物质。在未交配雌蝇中异位表达的Acp26Aa不会被加工处理。与精液发生改变的雄蝇交配的野生型雌蝇体内，Acp26Aa的加工取决于雄性附腺分泌物的存在和数量。Acp26Aa加工过程中对两性之间分子协作的需求可能会将其活性限制在交配后的雌蝇体内。
