Herndon L A, Wolfner M F
Section of Genetics and Development, Cornell University, Ithaca, NY 14853-2703, USA.
Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10114-8. doi: 10.1073/pnas.92.22.10114.
Mating triggers behavioral and physiological changes in the Drosophila melanogaster female, including an elevation of egg laying. Seminal fluid molecules from the male accessory gland are responsible for initial behavioral changes, but persistence of these changes requires stored sperm. Using genetic analysis, we have identified a seminal fluid protein that is responsible for an initial elevation of egg laying. This molecule, Acp26Aa, has structural features of a prohormone and contains a region with amino acid similarity to the egg-laying hormone of Aplysia. Acp26Aa is transferred to the female during mating, where it undergoes processing. Here we report the generation and analysis of mutants, including a null, in Acp26Aa. Females mated to male flies that lack Acp26Aa lay fewer eggs than do mates of normal males. This effect is apparent only on the first day after mating. The null mutation has no other detectable physiological or behavioral effects on the male or the mated female.
交配会引发黑腹果蝇雌性的行为和生理变化，包括产卵量增加。来自雄性附腺的精液分子负责引发最初的行为变化，但这些变化的持续需要储存的精子。通过基因分析，我们鉴定出一种负责最初产卵量增加的精液蛋白。这种分子，即Acp26Aa，具有前激素的结构特征，并且包含一个与海兔产卵激素具有氨基酸相似性的区域。Acp26Aa在交配过程中转移到雌性体内，并在那里进行加工处理。在此我们报告了Acp26Aa突变体的产生和分析，包括一个无效突变体。与缺乏Acp26Aa的雄蝇交配的雌蝇比与正常雄蝇交配的雌蝇产卵少。这种影响仅在交配后的第一天明显。无效突变对雄性或与之交配的雌性没有其他可检测到的生理或行为影响。
