大鼠胃黏膜损伤愈合过程中角质形成细胞生长因子和肝细胞生长因子的基因表达
Gene expression of keratinocyte and hepatocyte growth factors during the healing of rat gastric mucosal lesions.
作者信息
Kinoshita Y, Nakata H, Hassan S, Asahara M, Kawanami C, Matsushima Y, Naribayashi-Inomoto Y, Ping C Y, Min D, Nakamura A
机构信息
Department of Medicine, Kobe University School of Medicine, Japan.
出版信息
Gastroenterology. 1995 Oct;109(4):1068-77. doi: 10.1016/0016-5085(95)90564-2.
BACKGROUND & AIMS: The factors that stimulate the growth of gastric mucosal cells during gastric mucosal healing are not completely understood. This study was designed to investigate the gene expression of keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) in the healing of gastric mucosal lesions.
METHODS
Northern blot analysis and reverse-transcription polymerase chain reaction were used to show HGF and KGF messenger RNA.
RESULTS
Transcripts of HGF and KGF were not shown in rat gastric mucosal epithelium but were found in submucosal and muscular layers under normal conditions. When acute gastric mucosal lesions were induced by indomethacin treatment, expression of HGF messenger RNA was augmented in submucosal, muscular, or serosal layers, whereas the transcript of KGF was not influenced. When rat gastric mucosal epithelial cell line RGM1 and rat gastric mucosal primary culture cells were incubated with HGF or KGF, their proliferation was enhanced.
CONCLUSIONS
The results showed increased gene expression of HGF together with constant production of KGF during gastric mucosal healing.
背景与目的
胃黏膜愈合过程中刺激胃黏膜细胞生长的因素尚未完全明确。本研究旨在探讨角质形成细胞生长因子（KGF）和肝细胞生长因子（HGF）在胃黏膜损伤愈合中的基因表达情况。
方法
采用Northern印迹分析和逆转录聚合酶链反应检测HGF和KGF信使核糖核酸。
结果
正常情况下，大鼠胃黏膜上皮中未检测到HGF和KGF转录本，但在黏膜下层和肌层中可检测到。用吲哚美辛诱导急性胃黏膜损伤后，黏膜下层、肌层或浆膜层中HGF信使核糖核酸表达增加，而KGF转录本不受影响。用HGF或KGF培养大鼠胃黏膜上皮细胞系RGM1和大鼠胃黏膜原代培养细胞后，细胞增殖增强。
结论
结果表明，胃黏膜愈合过程中HGF基因表达增加，而KGF持续产生。
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