Ishibashi T, Shinogami M, Ishimoto S I, Yoshida K, Kaga K
Department of Otolaryngology, Tokyo University Branch Hospital, Japan.
Acta Otolaryngol. 1998 Sep;118(5):701-4. doi: 10.1080/00016489850183214.
KGF (KGF), synthesized and secreted exclusively by stromal cells in epithelialized organs, specifically promotes proliferation of cells of epithelial origin, including keratinocytes. A related peptide, basic fibroblast growth factor (bFGF), has mitogenic properties for fibroblasts and endothelial cells. KGF expression is stimulated markedly in the skin during wound healing. To investigate the physiologic action of KGF in the healing of TM (TM) perforations, we examined KGF and KGF receptor (KGFR) mRNA transcript levels as well as those of bFGF and transforming growth factor-alpha (TGFalpha) in normal and wounded rat TM at varying intervals, using a semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). We found KGF and TGFalpha mRNA expression to be induced rapidly, peaking 3 days after wounding and then declining. Expression of bFGF was induced gradually and remained increased until 7 days. In contrast, we found KGFR to be expressed in normal TM, remaining unchanged during TM repair. These results indicate that KGF and TGFalpha may mediate migration and proliferation of epithelial cells of the outer layer in the early stage of TM repair while bFGF may mediate the connective tissue reaction in the middle layer in a subsequent stage.
角质形成细胞生长因子（KGF）仅由上皮化器官中的基质细胞合成并分泌，特别能促进包括角质形成细胞在内的上皮来源细胞的增殖。一种相关肽，即碱性成纤维细胞生长因子（bFGF），对成纤维细胞和内皮细胞具有促有丝分裂特性。在伤口愈合过程中，皮肤中的KGF表达会显著上调。为了研究KGF在鼓膜（TM）穿孔愈合中的生理作用，我们使用半定量逆转录聚合酶链反应（RT-PCR），在不同时间间隔检测正常和受伤大鼠TM中KGF及其受体（KGFR）的mRNA转录水平，以及bFGF和转化生长因子-α（TGFα）的mRNA转录水平。我们发现KGF和TGFα的mRNA表达迅速被诱导，在受伤后3天达到峰值，然后下降。bFGF的表达逐渐被诱导，直到7天一直保持升高。相比之下，我们发现KGFR在正常TM中表达，在TM修复过程中保持不变。这些结果表明，KGF和TGFα可能在TM修复早期介导外层上皮细胞的迁移和增殖，而bFGF可能在随后阶段介导中层的结缔组织反应。
