伴有局灶性癌的大肠腺瘤DNA含量的流式细胞术分析

Flow cytometric analysis of the DNA content in colorectal adenomas with focal cancers.

作者信息

Suzuki S, Mizuno M, Tomoda J, Ohmori M, Tsuji T

机构信息

First Department of Internal Medicine, Okayama University Medical School, Japan.

出版信息

Gastroenterology. 1995 Oct;109(4):1098-104. doi: 10.1016/0016-5085(95)90567-7.

DOI:10.1016/0016-5085(95)90567-7

PMID:7557074

Abstract

BACKGROUND & AIMS: Variable results have been reported on the nuclear DNA content of colorectal polyps. The significance of DNA aneuploidy in the malignant transformation of colorectal polyps was evaluated.

METHODS

We analyzed by flow cytometry the nuclear DNA content of freshly frozen samples of 50 colorectal adenomas with or without focal cancers, analyzing separately the adenomatous and cancerous regions of the polyps.

RESULTS

In the adenomatous regions of the 50 polyps, the DNA was diploid in 43 and aneuploid in 7; the adenomas with DNA aneuploidy in the adenomatous regions were more frequently accompanied by focal cancers than were the DNA-diploid adenomas (P < 0.01). In 60% of the polyps with DNA aneuploidy in the cancerous regions, the DNA was also aneuploid in the adenomatous region and had similar DNA indices; this result suggests that the DNA aneuploidy had already occurred during the adenomatous stage, which lends support to the concept of the adenoma-carcinoma sequence. DNA aneuploidy in the adenomatous region was significantly correlated with the size of colorectal polyps (P < 0.05).

CONCLUSIONS

DNA aneuploidy may be an important indicator for the early diagnosis of malignant transformation of colorectal polyps.

摘要

背景与目的

关于结直肠息肉核DNA含量的报道结果不一。本研究评估了DNA非整倍体在结直肠息肉恶变中的意义。

方法

我们采用流式细胞术分析了50例伴有或不伴有局灶性癌的结直肠腺瘤新鲜冰冻样本的核DNA含量，并分别分析了息肉的腺瘤区和癌区。

结果

在50个息肉的腺瘤区，43个为DNA二倍体，7个为非整倍体；腺瘤区DNA非整倍体的腺瘤比DNA二倍体腺瘤更常伴有局灶性癌（P<0.01）。在癌区DNA非整倍体的息肉中，60%的腺瘤区DNA也为非整倍体且具有相似的DNA指数；这一结果表明DNA非整倍体在腺瘤阶段就已出现，支持腺瘤-癌序列的概念。腺瘤区DNA非整倍体与结直肠息肉大小显著相关（P<0.05）。