Aisaka K, Masuda T
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
FEMS Microbiol Lett. 1995 Aug 15;131(1):47-51. doi: 10.1016/0378-1097(95)00233-u.
A range of microorganisms was screened for new and high producer strains of trehalose phosphorylase (EC 2.4.1.64). Trehalose phosphorylase activity was found in cells of actinomyctes of the genera Actinomadura, Amycolata, Catellatospora, Kineosporia, and Nocardia. Among them, Catellatospora ferruginea showed the highest enzyme activity. Trehalose phosphorylase from C. ferruginea was able to catalyse both the phosphorolysis of trehalose into beta-glucose 1-phosphate and D-glucose and the synthesis of trehalose from beta-glucose 1-phosphate and D-glucose.
对一系列微生物进行了筛选，以寻找海藻糖磷酸化酶（EC 2.4.1.64）的新的高产菌株。在马杜拉放线菌属、无枝菌酸菌属、孢囊链霉菌属、动孢囊菌属和诺卡氏菌属的放线菌细胞中发现了海藻糖磷酸化酶活性。其中，铁锈色孢囊链霉菌表现出最高的酶活性。来自铁锈色孢囊链霉菌的海藻糖磷酸化酶能够催化海藻糖磷酸解为β-葡萄糖1-磷酸和D-葡萄糖，以及由β-葡萄糖1-磷酸和D-葡萄糖合成海藻糖。
