Pajni S, Chowdhury N R, Ghosh A, Kar S, Ghosh R K
Institute of Microbial Technology, Chandigarh, India.
FEMS Microbiol Lett. 1995 Aug 15;131(1):69-74. doi: 10.1111/j.1574-6968.1995.tb07756.x.
A temperate bacteriophage isolated from Vibrio cholerae O139, the new epidemic strain of cholera, was found to have a polyhedral head 65 nm in diameter and a rigid contractile tail 120 nm in length. The phage chromosome was a double-stranded DNA of 35 kb, with unique cohesive ends and had a G + C content of 58.8%. A restriction map of the phage DNA was constructed using the restriction endonucleases AvaI and BstEII. The phage, whose presence could be detected in nine out of 13 V. cholerae O139 isolates tested, was found to have identical chromosomal integration sites in all the strains. The phage attachment site (attP) was found to be located very close to one end of the genome.
从霍乱新流行菌株霍乱弧菌O139中分离出的一种温和噬菌体，其头部呈多面体，直径65纳米，尾部为刚性收缩尾，长度为120纳米。噬菌体染色体是一条35千碱基对的双链DNA，具有独特的粘性末端，G+C含量为58.8%。使用限制性内切酶AvaI和BstEII构建了噬菌体DNA的限制性图谱。在测试的13株霍乱弧菌O139分离株中，有9株能检测到该噬菌体，发现它在所有菌株中具有相同的染色体整合位点。噬菌体附着位点（attP）位于基因组一端附近。
