Thornewell S J, Peery R B, Skatrud P L
Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285, USA.
Gene. 1995 Aug 30;162(1):105-10. doi: 10.1016/0378-1119(95)00312-t.
The gene (TEF1) encoding translation elongation factor 1 alpha from the dimorphic fungus Aureobasidium pullulans (Ap) strain R106 has been cloned using Candida albicans TEF1 as a heterologous hybridization probe. Electrophoretic molecular karyotype/hybridization analysis of Ap revealed eight chromosomal bands and suggested that TEF1 resides on chromosome VI. Comparison of the genomic DNA sequence with the cDNA sequence of TEF1 verified the presence of three introns, the first one occurring five nucleotides from the start of translation. The deduced amino acid (aa) sequence encoded a protein consisting of 459 aa (49,663 Da) possessing high identity to a variety of TEF1-encoded proteins. A strong codon bias, similar to that observed in highly expressed yeast genes, was evident in A. pullulans TEF1. The ApTEF1 promoter region showed some sequence similarity to the well-studied TEF1 promoter from Saccharomyces cerevisiae, including a region from -23 to -11. This region exhibited high homology to a promoter upstream activating sequence (UAS) in S. cerevisiae. Such sequences have been shown to be essential promoter elements in genes coding for the highly expressed components of the yeast translation apparatus.
利用白色念珠菌的TEF1作为异源杂交探针，克隆了双态真菌出芽短梗霉（Ap）菌株R106中编码翻译延伸因子1α的基因（TEF1）。对Ap进行的电泳分子核型/杂交分析显示有八条染色体带，并表明TEF1位于第六条染色体上。将TEF1的基因组DNA序列与cDNA序列进行比较，证实存在三个内含子，第一个内含子位于翻译起始点的五个核苷酸处。推导的氨基酸（aa）序列编码一个由459个氨基酸（49,663道尔顿）组成的蛋白质，该蛋白质与多种TEF1编码的蛋白质具有高度同源性。在出芽短梗霉TEF1中明显存在强烈的密码子偏好，类似于在高表达酵母基因中观察到的情况。ApTEF1启动子区域与已深入研究的酿酒酵母TEF1启动子显示出一些序列相似性，包括从-23到-11的区域。该区域与酿酒酵母中的启动子上游激活序列（UAS）具有高度同源性。这些序列已被证明是编码酵母翻译装置高表达成分的基因中的必需启动子元件。
