粗糙脉孢菌met-10+基因座序列：与酿酒酵母8号染色体上一个功能未知序列的同源性

Sequence of the met-10+ locus of Neurospora crassa: homology to a sequence of unknown function in Saccharomyces cerevisiae chromosome 8.

作者信息

Chow C M, Kang S, Metzenberg R L, RajBhandary U L

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

出版信息

Gene. 1995 Aug 30;162(1):111-5. doi: 10.1016/0378-1119(95)00353-8.

DOI:10.1016/0378-1119(95)00353-8

PMID:7557397

Abstract

We have determined the sequence of the Neurospora crassa met-10+ gene and its flanking regions, and have isolated and analyzed cDNA clones for this region. We have identified two closely linked genes transcribed in the same orientation. The met-10+ gene is the downstream gene; an open reading frame (ORF) derived from five exons encodes a 475-amino-acid protein. The deduced protein lacks similarity to other characterized proteins. However, it exhibits a strong similarity to the product of an ORF of unknown function on Saccharomyces cerevisiae chromosome 8. This sequence similarity suggests functional equivalence and should facilitate identification of the function of met-10+ using gene disruptions in S. cerevisiae.

摘要

我们已经确定了粗糙脉孢菌met-10+基因及其侧翼区域的序列，并分离和分析了该区域的cDNA克隆。我们鉴定出两个紧密连锁且转录方向相同的基因。met-10+基因是下游基因；一个由五个外显子组成的开放阅读框（ORF）编码一种475个氨基酸的蛋白质。推导的蛋白质与其他已鉴定的蛋白质没有相似性。然而，它与酿酒酵母8号染色体上一个功能未知的ORF的产物具有很强的相似性。这种序列相似性表明功能等同，应该有助于通过酿酒酵母中的基因破坏来鉴定met-10+的功能。