Fosså A, Beyer A, Pfitzner E, Wenzel B, Kunau W H
Institut für Physiologische Chemie, Abteilung für Zellbiochemie, Ruhr-Universität Bochum, Germany.
Mol Gen Genet. 1995 Apr 10;247(1):95-104. doi: 10.1007/BF00425825.
We present the molecular cloning and sequencing of genomic and cDNA clones of the fox-2 gene of Neurospora crassa, encoding the multifunctional beta-oxidation protein (MFP). The coding region of the fox-2 gene is interrupted by three introns, one of which appears to be inefficiently spliced out. The encoded protein comprises 894 amino acid residues and exhibits 45% and 47% sequence identity with the MFPs of Candida tropicalis and Saccharomyces cerevisiae, respectively. Sequence analysis identifies three regions of the fungal MFPs that are highly conserved. These regions are separated by two segments that resemble linkers between domains of other MFPs, suggesting a three-domain structure. The first and second conserved regions of each MFP are homologous to each other and to members of the short-chain alcohol dehydrogenase family. We discuss these homologies in view of recent findings that fungal MFPs contain enoyl-CoA hydratase 2 and D-3-hydroxyacyl-CoA dehydrogenase activities, converting trans-2-enoyl-CoA via D-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. In contrast to its counterparts in yeasts, the Neurospora MFP does not have a C-terminal sequence resembling the SKL motif involved in protein targeting to microbodies.
我们展示了粗糙脉孢菌fox - 2基因的基因组和cDNA克隆的分子克隆及测序结果，该基因编码多功能β - 氧化蛋白（MFP）。fox - 2基因的编码区被三个内含子打断，其中一个内含子的剪接效率似乎较低。编码的蛋白质由894个氨基酸残基组成，与热带假丝酵母和酿酒酵母的MFP分别具有45%和47%的序列同一性。序列分析确定了真菌MFP的三个高度保守区域。这些区域被两个类似于其他MFP结构域之间连接子的片段隔开，表明其具有三结构域结构。每个MFP的第一和第二保守区域彼此同源，且与短链醇脱氢酶家族成员同源。鉴于最近的研究发现真菌MFP含有烯酰辅酶A水合酶2和D - 3 - 羟酰辅酶A脱氢酶活性，可将反式 - 2 - 烯酰辅酶A通过D - 3 - 羟酰辅酶A转化为3 - 酮酰辅酶A，我们讨论了这些同源性。与酵母中的对应物不同，粗糙脉孢菌的MFP没有类似于参与蛋白质靶向微体的SKL基序的C末端序列。
