棕色固氮菌recF基因的分子特征

Molecular characterization of the Azotobacter vinelandii recF gene.

作者信息

Badran H, Venkatesh T V, Kunnimalaiyaan M, Sharma N, Das H K

机构信息

Genetic Engineering Unit, Jawaharlal Nehru University, New Delhi, India.

出版信息

Gene. 1995 Aug 30;162(1):47-51. doi: 10.1016/0378-1119(95)00307-r.

DOI:10.1016/0378-1119(95)00307-r

PMID:7557415

Abstract

The recF gene from Azotobacter vinelandii (Av) has been cloned by complementation in an Escherichia coli (Ec) recF mutant. The sequence of 1568 bp has been determined and analyzed. It showed an open reading frame of 1092 nt coding for a 364-amino-acid (aa) polypeptide. The comparison of the deduced aa sequence of the recF of Av with those of other bacteria has elicited the presence of the four conserved domains thought to be essential for RecF function. A transcriptional fusion of a DNA fragment containing the promoter sequence of recF with the lacZ gene of Ec was constructed and 3-4-fold enhancement of promoter activity was observed upon UV induction.

摘要

通过在大肠杆菌（Ec）recF突变体中进行互补，克隆了来自棕色固氮菌（Av）的recF基因。已测定并分析了1568 bp的序列。它显示出一个1092 nt的开放阅读框，编码一个364个氨基酸（aa）的多肽。将棕色固氮菌recF推导的氨基酸序列与其他细菌的序列进行比较，发现了四个被认为对RecF功能至关重要的保守结构域。构建了一个包含recF启动子序列的DNA片段与大肠杆菌lacZ基因的转录融合体，在紫外线诱导后观察到启动子活性增强了3 - 4倍。