Venkatesh T V, Das H K
Genetic Engineering Unit, Jawaharlal Nehru University, New Delhi, India.
Gene. 1992 Apr 1;113(1):47-53. doi: 10.1016/0378-1119(92)90668-f.
The nucleotide (nt) sequence of the Azotobacter vinelandii recA gene (Av-recA) was determined and compared with the recA sequences from Pseudomonas aeruginosa (Pa-recA), a soil bacterium, and Escherichia coli (Ec-recA), an enteric bacterium. The Av-recA gene and the deduced aa sequence were found to be more similar to their Pa-recA counterparts than to the Ec-recA gene and protein. Expression of Av-recA was found to be autoregulatory. Unlike Ec-recA and Pa-recA, however, expression of Av-recA was weakly enhanced upon DNA damage. In E. coli, expression of an Av-recA::lacZ fusion was poor, but its autoregulation was similar to that of Ec-recA. Av-recA expression, however, could not induce the repair system response in E. coli.
测定了棕色固氮菌recA基因（Av-recA）的核苷酸（nt）序列，并将其与来自土壤细菌铜绿假单胞菌（Pa-recA）和肠道细菌大肠杆菌（Ec-recA）的recA序列进行了比较。发现Av-recA基因及其推导的氨基酸序列与其Pa-recA对应序列的相似性高于与Ec-recA基因和蛋白质的相似性。发现Av-recA的表达是自动调节的。然而，与Ec-recA和Pa-recA不同，DNA损伤后Av-recA的表达仅微弱增强。在大肠杆菌中，Av-recA::lacZ融合蛋白的表达较差，但其自动调节与Ec-recA相似。然而，Av-recA的表达不能诱导大肠杆菌中的修复系统反应。
