牛白细胞介素-3编码cDNA的克隆

Cloning of the bovine interleukin-3-encoding cDNA.

作者信息

Mwangi S M, Logan-Henfrey L, McInnes C, Mertens B

机构信息

International Livestock Research Institute (ILRI), Nairobi, Kenya.

出版信息

Gene. 1995 Sep 11;162(2):309-12. doi: 10.1016/0378-1119(95)00359-e.

DOI:10.1016/0378-1119(95)00359-e

PMID:7557449

Abstract

Interleukin-3 (IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared from RNA isolated from 4- and 5-hour concanavalin-A-stimulated peripheral blood lymphocytes (PBL) from N'Dama cattle. An analysis of the cDNA sequence reveals that it contains a 432-nucleotide (nt) open reading frame which codes for 144 amino acids (aa). Cleavage of the putative signal peptide consisting of the first 17 aa yields the mature form of the protein (14.5 kDa). Comparisons of the bovine IL-3 sequence with the sheep, human and mouse IL-3 sequences show that the bovine sequence shares 90.7, 55.8 and 51.9% nt identity, respectively, in the coding region, and 85.4, 35 and 27.7% aa identity, respectively.

摘要

白细胞介素-3（IL-3）是在血细胞形成的早期和晚期发挥作用的细胞因子之一。为了研究IL-3在牛造血干细胞分化中的作用，采用聚合酶链反应从取自恩达马牛经伴刀豆球蛋白A刺激4小时和5小时的外周血淋巴细胞（PBL）分离的RNA制备的第一链cDNA中扩增出IL-3 cDNA。对该cDNA序列的分析表明，它包含一个432个核苷酸（nt）的开放阅读框，编码144个氨基酸（aa）。由最初17个氨基酸组成的假定信号肽的切割产生了该蛋白质的成熟形式（14.5 kDa）。牛IL-3序列与绵羊、人类和小鼠IL-3序列的比较表明，牛序列在编码区的核苷酸同一性分别为90.7%、55.8%和51.9%，氨基酸同一性分别为85.4%、35%和27.7%。