Giménez A, Hostench J, Stamatoglou S C, Enrich C
Liver Unit, Fundació Clínic, Hospital Clínic i Provincial de Barcelona, Spain.
Hepatology. 1995 Oct;22(4 Pt 1):1259-62.
We have studied the distribution and expression of laminin during rat liver regeneration by immunofluorescence and immunoblotting using affinity-purified laminin antibodies. Laminin was localized on sinusoidal surfaces in normal and regenerating hepatic parenchyma, but enhanced expression was detected during regeneration from 6 hours to 7 days after a partial hepatectomy. A and B laminin chains were specifically detected by Western blotting in highly purified plasma membrane fractions derived from the sinusoidal domain of hepatocytes. Analysis of laminin expression in whole homogenates from hepatectomized rats showed a differential expression of A and B laminin chains during regeneration: The A chain showed a progressive increase, whereas the B chain exhibited an early, prereplicative increase and then gradually declined to control levels at the end of restorative growth. The results suggest differential temporal requirements for A and B laminin chains during hepatic growth and tissue formation.
我们使用亲和纯化的层粘连蛋白抗体，通过免疫荧光和免疫印迹法研究了大鼠肝脏再生过程中层粘连蛋白的分布和表达。层粘连蛋白定位于正常和再生肝实质的窦状表面，但在部分肝切除术后6小时至7天的再生过程中检测到表达增强。通过蛋白质免疫印迹法在源自肝细胞窦状结构域的高度纯化的质膜组分中特异性检测到A和B层粘连蛋白链。对肝切除大鼠全匀浆中层粘连蛋白表达的分析表明，再生过程中A和B层粘连蛋白链存在差异表达：A链呈逐渐增加，而B链在复制前期早期增加，然后在恢复性生长结束时逐渐降至对照水平。结果表明，在肝脏生长和组织形成过程中，A和B层粘连蛋白链存在不同的时间需求。
