Anilkumar N, Sudhakaran P R
Dept of Biochemistry, University of Kerala, Kariavattom Trivandrum, India.
Biochem Mol Biol Int. 1993 Oct;31(2):201-9.
Interaction of rat hepatocytes with laminin, the major basement membrane adhesive glycoprotein was studied. Rat fetal hepatocytes attached more to laminin than adult hepatocytes. Laminin promoted attachment of fetal hepatocytes in a concentration dependent manner and showed saturable binding pattern. Hepatocytes from CCl4 induced regenerating rat liver also attached more to laminin. The pentapeptide YIGSR derived from laminin promoted attachment of adult and fetal hepatocytes, but to a lesser extent. A 67 kDa protein was isolated from the hepatic plasma membrane of CCl4 induced regenerating rat liver by affinity chromatography over laminin sepharose. This protein appeared to be relatively abundant in regenerating liver than in normal liver. The radioiodinated 67 kDa protein could be inserted into liposomes and these liposomes attached to coverslips coated with laminin in a concentration dependent manner in a divalent cation free medium. Its specificity for laminin was also revealed by absence of significant binding to fibronectin and collagen.
研究了大鼠肝细胞与层粘连蛋白（主要的基底膜黏附糖蛋白）之间的相互作用。大鼠胎儿肝细胞比成年肝细胞更易附着于层粘连蛋白。层粘连蛋白以浓度依赖方式促进胎儿肝细胞的附着，并呈现出饱和结合模式。四氯化碳诱导的大鼠再生肝中的肝细胞也更易附着于层粘连蛋白。源自层粘连蛋白的五肽YIGSR促进成年和胎儿肝细胞的附着，但程度较小。通过层粘连蛋白琼脂糖亲和层析从四氯化碳诱导的大鼠再生肝的肝细胞膜中分离出一种67 kDa的蛋白质。该蛋白质在再生肝中似乎比正常肝中更为丰富。放射性碘化的67 kDa蛋白质可插入脂质体，并且这些脂质体在无二价阳离子的培养基中以浓度依赖方式附着于涂有层粘连蛋白的盖玻片上。其对层粘连蛋白的特异性还通过与纤连蛋白和胶原蛋白无明显结合得以体现。
