Mohs F E
Head Neck Surg. 1978 Nov-Dec;1(2):150-66. doi: 10.1002/hed.2890010209.
Cancer of the skin and of other accessible structures can be removed under complete microscopic control if the cancerous area is excised layer by layer, and if the undersurface of each layer is then examined under the microscope through systematic use of frozen sections. If the cancer is extensive and complicated, or if the neoplasm is of a type that is readily disseminated, the tissues are fixed in situ with zinc chloride prior to the excision of each layer (fixed-tissue technique). If the cancer is not overly extensive or complicated, the layers of tissue are excised in the fresh, unfixed state (fresh-tissue technique). Both methods are highly reliable. In two consecutive series of basal-cell carcinomas treated chemosurgically, the five-year cure rate achieved was 99.3% for the 9,351 lesions removed by the fixed-tissue technique, and 98.1% for the 196 lesions removed by the fresh-tissue method.
如果癌灶逐层切除，且每层的底面随后通过系统使用冰冻切片在显微镜下进行检查，那么皮肤癌及其他体表结构的癌症能够在完全的显微镜控制下被切除。如果癌症范围广泛且复杂，或者肿瘤属于易于扩散的类型，那么在每层切除之前，先用氯化锌将组织原位固定（固定组织技术）。如果癌症并非过于广泛或复杂，组织层则在新鲜、未固定的状态下切除（新鲜组织技术）。这两种方法都高度可靠。在连续两组接受化学外科治疗的基底细胞癌病例中，采用固定组织技术切除的9351个病灶的五年治愈率为99.3%，采用新鲜组织方法切除的196个病灶的五年治愈率为98.1%。
