Collin O, Kilter S, Bergh A
Department of Anatomy, University of Umeåa, Sweden.
Int J Androl. 1995 Jun;18(3):141-5. doi: 10.1111/j.1365-2605.1995.tb00402.x.
Anaesthetized adult rats were exposed repeatedly to cigarette smoke for 2 s interspersed with exposure to fresh air for either 10, 15 or 30 s using a smoking apparatus. The acute effects of this treatment on testicular microcirculation were studied using laser Doppler flowmetry. Peripheral tissue O2 saturation was measured continuously in the foot during the experiment. Exposure to cigarette smoke caused a moderate acute increase in the testicular blood flow and it inhibited vasomotion. Blood flow and the pattern of vasomotion were normalized after termination of smoke exposure. The time between the last smoke exposure and flow normalization was dependent on the smoke/air dose. Peripheral tissue O2 saturation did not change during the experiments. The effects on testicular microcirculation of local injections of an aqueous extract of cigarette smoke and passive smoking were also studied. Local injection of smoke extract increased blood flow and inhibited vasomotion. Passive smoking caused a moderate decrease in the frequency of vasomotion and amplitude. The observation that cigarette smoke influences testicular microcirculation acutely is discussed in relation to possible adverse effects on spermatogenesis.
使用吸烟装置，将麻醉的成年大鼠反复暴露于香烟烟雾中2秒，期间穿插暴露于新鲜空气中10秒、15秒或30秒。使用激光多普勒血流仪研究了这种处理对睾丸微循环的急性影响。实验过程中持续测量足部外周组织的氧饱和度。暴露于香烟烟雾会使睾丸血流量适度急性增加，并抑制血管运动。停止烟雾暴露后，血流量和血管运动模式恢复正常。最后一次烟雾暴露与血流恢复正常之间的时间取决于烟雾/空气剂量。实验过程中外周组织的氧饱和度没有变化。还研究了局部注射香烟烟雾水提取物和被动吸烟对睾丸微循环的影响。局部注射烟雾提取物会增加血流量并抑制血管运动。被动吸烟会使血管运动的频率和幅度适度降低。结合对精子发生可能产生的不利影响，讨论了香烟烟雾急性影响睾丸微循环这一观察结果。
