由乙酰化甘露聚糖（一种从库拉索芦荟中提取的复合碳水化合物）激活的鸡巨噬细胞产生一氧化氮。

Nitric oxide production by chicken macrophages activated by Acemannan, a complex carbohydrate extracted from Aloe vera.

作者信息

Karaca K, Sharma J M, Nordgren R

机构信息

University of Minnesota, College of Veterinary Medicine, Department of Pathobiology, St Paul 55108, USA.

出版信息

Int J Immunopharmacol. 1995 Mar;17(3):183-8. doi: 10.1016/0192-0561(94)00102-t.

DOI:10.1016/0192-0561(94)00102-t

PMID:7558512

Abstract

Cultures of normal chicken spleen cells and HD11 line cells produce nitric oxide (NO) in response to Acemannan, a complex carbohydrate derived from the Aloe vera plant. Neither cell type produced detectable amounts of NO in response to similar concentrations of yeast mannan, another complex carbohydrate. Nitric oxide production was dose dependent and inhibitable by the nitric oxide synthase inhibitor NG-methyl-L-arginine. In addition, the production of NO was inhibited by preincubation of ACM with concanavalin A in a dose-dependent manner. These results suggest that ACM-induced NO synthesis may be mediated through macrophage mannose receptors, and macrophage activation may be accountable for some of the immunomodulatory effects of ACM in chickens.

摘要

正常鸡脾细胞和HD11系细胞的培养物在响应乙酰化甘露聚糖（一种源自芦荟植物的复合碳水化合物）时会产生一氧化氮（NO）。两种细胞类型在响应相似浓度的酵母甘露聚糖（另一种复合碳水化合物）时均未产生可检测量的NO。一氧化氮的产生呈剂量依赖性，且可被一氧化氮合酶抑制剂NG-甲基-L-精氨酸抑制。此外，用伴刀豆球蛋白A预孵育乙酰化甘露聚糖（ACM）可剂量依赖性地抑制NO的产生。这些结果表明，ACM诱导的NO合成可能通过巨噬细胞甘露糖受体介导，巨噬细胞激活可能是ACM对鸡的某些免疫调节作用的原因。