Zhang L, Tizard I R
Department of Veterinary Pathobiology, Texas A & M University College Station 77843, USA.
Immunopharmacology. 1996 Nov;35(2):119-28. doi: 10.1016/s0162-3109(96)00135-x.
Acemannan is the name given to the major carbohydrate fraction obtained from the gel of the Aloe vera leaf. It has been claimed to have several important therapeutic properties including acceleration of wound healing, immune stimulation, anti-cancer and anti-viral effects. However, the biological mechanisms of these activities are unclear. Because of this wide diversity of effects, it is believed that they may be exerted through pluripotent effector cells such as macrophages. The effects of acemannan on the mouse macrophage cell line, RAW 264.7 cells were therefore investigated. It was found that acemannan could stimulate macrophage cytokine production, nitric oxide release, surface molecule expression, and cell morphologic changes. The production of the cytokines IL-6 and TNF-alpha were dependent on the dose of acemannan provided. Nitric oxide production, cell morphologic changes and surface antigen expression were increased in response to stimulation by a mixture of acemannan and IFN-gamma. These results suggest that acemannan may function, at least in part, through macrophage activation.
乙酰甘露聚糖是从库拉索芦荟叶凝胶中提取的主要碳水化合物部分的名称。据称它具有多种重要的治疗特性，包括加速伤口愈合、免疫刺激、抗癌和抗病毒作用。然而，这些活性的生物学机制尚不清楚。由于其作用的多样性，人们认为它们可能是通过多能效应细胞如巨噬细胞发挥作用的。因此，研究了乙酰甘露聚糖对小鼠巨噬细胞系RAW 264.7细胞的影响。发现乙酰甘露聚糖可以刺激巨噬细胞细胞因子的产生、一氧化氮的释放、表面分子的表达以及细胞形态的变化。细胞因子IL-6和TNF-α的产生取决于所提供的乙酰甘露聚糖的剂量。乙酰甘露聚糖和IFN-γ的混合物刺激后，一氧化氮的产生、细胞形态变化和表面抗原表达增加。这些结果表明，乙酰甘露聚糖可能至少部分通过巨噬细胞激活发挥作用。
