Tao Yuheng, Wang Ting, Lai Chenhuan, Ling Zhe, Zhou Yanmin, Yong Qiang
Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China.
Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, China.
Front Bioeng Biotechnol. 2021 Apr 9;9:679558. doi: 10.3389/fbioe.2021.679558. eCollection 2021.
As known, the nutritional status affects antioxidant capacity and immunity, ultimately affecting the body's health. Recently, hemicellulosic polysaccharides of galactomannan in different biomass and their degradation products are gaining more attention due to excellent antioxidant enhancement and immunomodulatory activity. Herein, incomplete degradation products of galactomannan (IDPG) were prepared from the enzymatic hydrolysis of seeds, followed by the and experiments. Using an HO-injured RAW264.7 cells model, IDPG was demonstrated to have antioxidant capacity, as indicated by superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. While in the evaluation in laying hens (68-weeks-old), diets were supplemented with 0, 0.01, 0.025, and 0.05% IDPG for 8 weeks, respectively. Our results showed that IDPG can improve antioxidant capacity by increasing antioxidants contents and reducing MDA content. Furthermore, IDPG can increase immunoglobulins and cytokines secretion, thereby enhancing the immunity of laying hens. This result was further demonstrated by experiment, in which IDPG significantly increased the secretion of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and toll-like receptor 4 (TLR4) in RAW264.7 cells ( < 0.05). Overall, IDPG can improve antioxidant function and modulate immunological response, thereby the concept of using IDPG for health may gain a little more credibility.
众所周知，营养状况会影响抗氧化能力和免疫力，最终影响身体健康。最近，不同生物质中半乳甘露聚糖的半纤维素多糖及其降解产物因其出色的抗氧化增强和免疫调节活性而受到越来越多的关注。在此，通过种子的酶水解制备了半乳甘露聚糖的不完全降解产物（IDPG），随后进行了[此处原文缺失相关实验内容]实验。使用过氧化氢损伤的RAW264.7细胞模型，超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量表明IDPG具有抗氧化能力。在对68周龄蛋鸡的评估中，分别在日粮中添加0、0.01、0.025和0.05%的IDPG，持续8周。我们的结果表明，IDPG可以通过增加抗氧化剂含量和降低MDA含量来提高抗氧化能力。此外，IDPG可以增加免疫球蛋白和细胞因子的分泌，从而增强蛋鸡的免疫力。RAW264.7细胞实验进一步证明了这一结果，其中IDPG显著增加了一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）、白细胞介素6（IL-6）和Toll样受体4（TLR4）的分泌（P<0.05）。总体而言，IDPG可以改善抗氧化功能并调节免疫反应，因此使用IDPG促进健康的概念可能更具可信度。
