一匹患有性腺发育不全的XY母马的分子分析。
Molecular analysis of an XY mare with gonadal dysgenesis.
作者信息
Pailhoux E, Cribiu E P, Parma P, Cotinot C
机构信息
INRA, Centre de Recherches de Jouy, Jouy-en-Josas, France.
出版信息
Hereditas. 1995;122(2):109-12. doi: 10.1111/j.1601-5223.1995.00109.x.
In this study, cytogenetic analysis of an infertile mare revealed a 64, XY karyotype. The XY sex-reversed animal had a female phenotype with gonadal dysgenesis. Using Southern blot analysis, we tested for the presence of two Y-specific genes SRY and ZFY by using DNA isolated from peripheral blood leukocytes. The results showed that at least the DNA-binding domain of the SRY gene was deleted from the Y chromosome of the XY mare but that the ZFY gene was present on this chromosome.
在本研究中，对一匹不育母马的细胞遗传学分析显示其核型为64，XY。这只XY性反转动物具有雌性表型且伴有性腺发育不全。我们使用从外周血白细胞中分离的DNA，通过Southern印迹分析检测了两个Y特异性基因SRY和ZFY的存在情况。结果表明，XY母马的Y染色体上至少缺失了SRY基因的DNA结合结构域，但ZFY基因存在于该染色体上。
Zotero 插件