Yi Q M, Deng W G, Xia Z P, Pang H H
Department of Biochemistry, Wuhan University, China.
Hereditas. 1995;122(2):135-41. doi: 10.1111/j.1601-5223.1995.00135.x.
We have applied the arbitrarily primed polymerase chain reaction (AP-PCR) technique to the analysis of the relationships among six japonica and indica cultivars, and four wild species of rice. Chosen were four primers of arbitrary sequence that gave multiple amplification products when rice DNA was used as template. Among a total of 50 bands scored, 44 were polymorphic, which was sufficient to distinguish the species used in this study. It is apparent from the comparisons of genetic distances that cultivated rice (Oryza sativa) exhibits closest molecular affinity to wild O. rufipogon, suggesting that the origin of cultivated rice is from O. rufipogon. In a dendrogram of cultivated rice and O. rufipogon from different regions, japonica and indica rice were most closely clustered with O. rufipogon from China and India, respectively. Japonica and indica subspecies showed closer affinity with O. rufipogon from different origins than with each other, supporting a hypothesis of multi-centers of the origin of rice cultivation.
我们已将任意引物聚合酶链反应（AP-PCR）技术应用于六个粳稻和籼稻品种以及四种野生稻之间亲缘关系的分析。选用了四个具有任意序列的引物，以水稻DNA为模板时可产生多个扩增产物。在总共记录的50条带中，有44条具有多态性，这足以区分本研究中使用的物种。从遗传距离的比较中可以明显看出，栽培稻（Oryza sativa）与野生稻O. rufipogon表现出最密切的分子亲缘关系，这表明栽培稻起源于O. rufipogon。在来自不同地区的栽培稻和O. rufipogon的聚类图中，粳稻和籼稻分别与来自中国和印度的O. rufipogon聚类最为紧密。粳稻和籼稻亚种与来自不同起源的O. rufipogon的亲缘关系比它们彼此之间的亲缘关系更近，这支持了水稻栽培起源多中心的假说。
