Levanon D, Stein H
Unit of Electron Microscopy, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Histochem J. 1995 Jun;27(6):457-65.
The ability of tannic acid to enhance binding of glycosaminoglycans to purified collagen was analysed in an in vitro system using amino sugar analysis on an amino acid analyser, transmission electron microscopy, and scanning electron microscopy. Collagen was purified by digestion with trypsin, papain, and hyaluronidase. Purified collagen was incubated with hyaluronic acid or with chondroitin sulphate glycosaminoglycan and then treated with tannic acid. Tannic acid was found to enhance retention during preparation for electron microscopy of either of the glycosaminoglycans onto collagen fibres. The ability of tannic acid to enhance binding of collagen and glycosaminoglycans might explain, at least in part, its structural reinforcement effect on resected synovial joint-apposing surfaces during preparation for scanning electron microscopy.
在体外系统中，利用氨基酸分析仪进行氨基糖分析、透射电子显微镜和扫描电子显微镜，分析了鞣酸增强糖胺聚糖与纯化胶原蛋白结合的能力。通过用胰蛋白酶、木瓜蛋白酶和透明质酸酶消化来纯化胶原蛋白。将纯化的胶原蛋白与透明质酸或硫酸软骨素糖胺聚糖一起孵育，然后用鞣酸处理。结果发现，鞣酸可增强在电子显微镜制备过程中任一糖胺聚糖在胶原纤维上的保留。鞣酸增强胶原蛋白和糖胺聚糖结合的能力，至少在一定程度上可以解释其在扫描电子显微镜制备过程中对切除的滑膜关节贴合面的结构增强作用。
