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鞣酸在制备兔膝关节半月板用于扫描电子显微镜检查中的应用评估。

Evaluation of the use of tannic acid in preparation of the rabbit knee meniscus for scanning electron microscopy.

作者信息

Levanon D, Stein H

机构信息

Unit of Electron Microscopy, Rambam Medical Center, Technion-Israel Institute of Technology, Haifa, Israel.

出版信息

Scanning Microsc. 1993 Jun;7(2):741-8; discussion 749-50.

PMID:9026907
Abstract

The femoral faces of medial and lateral menisci of the rabbit knee were structurally reinforced with tannic acid and studied using the scanning electron microscope. Significant improvement in preservation of the meniscal surfaces, as compared to previous studies, was brought about by the structural reinforcement technique. Menisci, although detacted from tibial plateaux, retained well their shape, dimensions, and microarchitecture. Differences in surface morphology between medial and lateral menisci and between longitudinal sectors on menisci were recorded. Appearances of surface irregulatities were reduced to a very low level, and the possibility that this minimum exists in situ is forwarded.

摘要

用单宁酸对兔膝关节内侧和外侧半月板的股骨面进行结构加固,并使用扫描电子显微镜进行研究。与先前的研究相比,结构加固技术显著改善了半月板表面的保存情况。半月板虽然从胫骨平台分离,但仍很好地保留了其形状、尺寸和微观结构。记录了内侧和外侧半月板之间以及半月板纵向区域之间的表面形态差异。表面不规则的外观减少到非常低的水平,并提出了这种最小值在原位存在的可能性。

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Evaluation of the use of tannic acid in preparation of the rabbit knee meniscus for scanning electron microscopy.鞣酸在制备兔膝关节半月板用于扫描电子显微镜检查中的应用评估。
Scanning Microsc. 1993 Jun;7(2):741-8; discussion 749-50.
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引用本文的文献

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Articular cartilage of the rabbit knee after synovectomy: a scanning electron microscopy study.滑膜切除术后兔膝关节的关节软骨:一项扫描电子显微镜研究。
J Anat. 1998 Apr;192 ( Pt 3)(Pt 3):343-9. doi: 10.1046/j.1469-7580.1998.19230343.x.
2
Quantitative analysis of chondroitin sulphate retention by tannic acid during preparation of specimens for electron microscopy.电子显微镜标本制备过程中单宁酸对硫酸软骨素保留情况的定量分析。
Histochem J. 1995 Jun;27(6):457-65.