人胃癌中细胞周期蛋白E基因的频繁扩增。
Frequent amplification of the cyclin E gene in human gastric carcinomas.
作者信息
Akama Y, Yasui W, Yokozaki H, Kuniyasu H, Kitahara K, Ishikawa T, Tahara E
机构信息
First Department of Pathology, Hiroshima University School of Medicine.
出版信息
Jpn J Cancer Res. 1995 Jul;86(7):617-21. doi: 10.1111/j.1349-7006.1995.tb02442.x.
We searched for genetic alterations of the cyclin D1 and cyclin E genes in 45 human gastric carcinoma tissues. Expression of cyclin E mRNA and protein was also analyzed in eight of them by Northern and Western blots and immunohistochemical staining. The cyclin E gene was amplified 3-10 fold in seven gastric cancer tissues (15.6%), of which six were advanced gastric cancers. All of the cases with the cyclin E gene amplification displayed lymph node metastasis. Moreover, the case with the gene amplification overexpressed the cyclin E mRNA and protein. One of eight gastric cancer cell lines, MKN-7, shared the cyclin E gene amplification, and all of the gastric cancer cell lines expressed high levels of the cyclin E mRNA and protein even without gene amplification. Amplification of the cyclin D1 gene was not observed in any of the gastric carcinoma tissues or gastric carcinoma cell lines. These results suggest that the gene amplification and overexpression of cyclin E play an important role in the abnormal growth and progression of gastric carcinoma.
我们对45例人胃癌组织中的细胞周期蛋白D1和细胞周期蛋白E基因的遗传改变进行了研究。通过Northern印迹法、Western印迹法和免疫组化染色对其中8例组织中的细胞周期蛋白E mRNA和蛋白表达情况也进行了分析。在7例胃癌组织（15.6%）中，细胞周期蛋白E基因扩增了3至10倍，其中6例为进展期胃癌。所有细胞周期蛋白E基因扩增的病例均出现淋巴结转移。此外，基因扩增的病例中细胞周期蛋白E mRNA和蛋白呈过表达。8株胃癌细胞系之一的MKN-7也存在细胞周期蛋白E基因扩增，并且所有胃癌细胞系即使在没有基因扩增的情况下也高水平表达细胞周期蛋白E mRNA和蛋白。在任何胃癌组织或胃癌细胞系中均未观察到细胞周期蛋白D1基因扩增。这些结果表明，细胞周期蛋白E的基因扩增和过表达在胃癌的异常生长和进展中起重要作用。
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