Gillott K L, Cox V M, Wright H, Eaves L A, Williams P E, Goldspink D F
Department of Medicine, University of Leeds, UK.
J Anat. 1994 Aug;185 ( Pt 1)(Pt 1):173-9.
The fibre type distribution has been mapped in the latissimus dorsi muscle of the Dutch rabbit. Using the myosin ATPase stain, a distinct border was found to run in a cranial to caudal direction, which effectively divided the muscle into 2 segments of different fibre type proportions. Although both segments contained mostly fast twitch fibres, the medial areas were found to contain approximately 10-20% slow (i.e. type I) fibres while the lateral portions contained very few, if any, slow fibres. Significantly fewer type IIa fibres were also found in the lateral areas of the muscle. These histochemical findings were confirmed by the use of the reverse transcriptase polymerase chain reaction, which demonstrated that more messenger RNA of the slow myosin heavy chain was found in the medial regions compared with the lateral segment. These results demonstrate the importance of choosing well defined sampling sites when evaluating regimes designed to transform this heterogeneous muscle for use in subsequent myoplasty procedures.
已绘制出荷兰兔背阔肌的纤维类型分布图。使用肌球蛋白ATP酶染色法，发现一条明显的边界从颅侧向尾侧延伸，这条边界有效地将肌肉分成了纤维类型比例不同的两个部分。虽然两个部分主要都含有快肌纤维，但发现内侧区域含有约10%-20%的慢肌纤维（即I型纤维），而外侧部分即使有慢肌纤维也非常少。在肌肉的外侧区域还发现IIa型纤维明显较少。这些组织化学结果通过逆转录聚合酶链反应得到了证实，该反应表明与外侧部分相比，在内侧区域发现了更多的慢肌球蛋白重链信使RNA。这些结果表明，在评估旨在改变这种异质性肌肉以用于后续肌成形术的方案时，选择明确界定的采样部位非常重要。
