Hämäläinen N, Pette D
Faculty of Biology, University of Konstanz, Federal Republic of Germany.
J Histochem Cytochem. 1993 May;41(5):733-43. doi: 10.1177/41.5.8468455.
This study characterized histochemically three fast fiber types (IIB, IID, IIA) in skeletal muscles of mouse, rat, and rabbit, with special reference to fiber types IIB and IID. The results are complemented by biochemical analyses of myosin heavy chain composition in these muscles. Fiber type delineation is based on various methods for mATPase staining with pre-incubations and assays under different conditions. In rat and mouse, IIB and IID fibers can be best distinguished according to their different mATPase stabilities towards formaldehyde and alkaline pH. In rabbit, the method of Matoba and Gollnick using acid pre-incubation provided best and most reproducible results. In addition to their different mATPase stabilities, the three fast fiber types differ with regard to their oxidative capacities and cross-sectional fiber areas in the three species. In general, Type IIB fibers are the largest and least oxidative, Type IIA fibers the smallest and most oxidative, and Type IID fibers intermediate. In rabbit, Type IID fibers are the predominant fast fiber population in extensor digitorum longus, psoas, and tibialis anterior muscles. As judged from histochemistry, these muscles of rabbit do not contain pure Type IIB fibers. This is in accordance with biochemical results that show the HCIId to form the majority of the myosin heavy chain complement expressed in these muscles. On the other hand, IIB fibers are numerous in rabbit adductor magnus, gastrocnemius, and vastus lateralis muscles. Similarly, appreciable amounts of myosin heavy chain HCIIb are found in the three latter muscles of rabbit.
本研究通过组织化学方法对小鼠、大鼠和兔子骨骼肌中的三种快肌纤维类型（IIB、IID、IIA）进行了表征，特别关注了IIB和IID纤维类型。这些结果通过对这些肌肉中肌球蛋白重链组成的生化分析得到了补充。纤维类型的划分基于在不同条件下进行预孵育和检测的多种mATPase染色方法。在大鼠和小鼠中，IIB和IID纤维可以根据它们对甲醛和碱性pH的不同mATPase稳定性得到最佳区分。在兔子中，Matoba和Gollnick使用酸预孵育的方法提供了最佳且最可重复的结果。除了不同的mATPase稳定性外，这三种快肌纤维类型在这三个物种中的氧化能力和纤维横截面积也有所不同。一般来说，IIB型纤维最大且氧化能力最低，IIA型纤维最小且氧化能力最高，IID型纤维介于两者之间。在兔子中，IID型纤维是趾长伸肌、腰大肌和胫骨前肌中主要的快肌纤维群体。从组织化学判断，兔子的这些肌肉中不含有纯的IIB型纤维。这与生化结果一致，生化结果显示HCIId构成了这些肌肉中表达的肌球蛋白重链的大部分。另一方面，IIB型纤维在兔子的大收肌、腓肠肌和股外侧肌中数量众多。同样，在兔子的后三种肌肉中也发现了相当数量的肌球蛋白重链HCIIb。
