Ojeda A, Prado V, Martinez J, Arellano C, Borczyk A, Johnson W, Lior H, Levine M M
Department of Microbiology, Faculty of Medicine, University of Chile, Santiago.
J Clin Microbiol. 1995 Aug;33(8):2199-201. doi: 10.1128/jcm.33.8.2199-2201.1995.
Enterohemorrhagic Escherichia coli (EHEC) strains detected with DNA probes (for virulence plasmid and Shiga-like toxins) from subjects with hemolytic-uremic syndrome (n = 19) or diarrhea (n = 41) or asymptomatic carriers (n = 29) were examined for sorbitol fermentability, as were enterotoxigenic (n = 40), enteropathogenic (n = 40), and enteroinvasive (n = 40) E. coli and urinary tract infection (n = 40) strains and normal flora E. coli strains (n = 40). Sorbitol negativity was common only in EHEC, particularly among strains from severe clinical infections. All 19 EHEC strains from patients with hemolytic-uremic syndrome, irrespective of O:H serotype or Shiga-like toxin genotype, were sorbitol negative.
使用DNA探针(针对毒力质粒和志贺样毒素)从溶血尿毒综合征患者(n = 19)、腹泻患者(n = 41)或无症状携带者(n = 29)中检测出的肠出血性大肠杆菌(EHEC)菌株,以及产肠毒素大肠杆菌(n = 40)、肠致病性大肠杆菌(n = 40)、肠侵袭性大肠杆菌(n = 40)、尿路感染菌株(n = 40)和正常菌群大肠杆菌菌株(n = 40),均接受了山梨醇发酵能力检测。山梨醇阴性仅在肠出血性大肠杆菌中常见,尤其是在严重临床感染的菌株中。所有19株来自溶血尿毒综合征患者的肠出血性大肠杆菌菌株,无论O:H血清型或志贺样毒素基因型如何,均为山梨醇阴性。