Levine M M, Xu J G, Kaper J B, Lior H, Prado V, Tall B, Nataro J, Karch H, Wachsmuth K
J Infect Dis. 1987 Jul;156(1):175-82. doi: 10.1093/infdis/156.1.175.
Enterohemorrhagic Escherichia coli (EHEC) cause hemorrhagic colitis and hemolytic uremic syndrome (HUS), make potent cytotoxins (Verotoxins [VT] or Shiga-like toxins), and possess a plasmid (approximately 60 megadaltons) that encodes a new fimbrial antigen and promotes attachment to epithelial cells. We evaluated the use of a DNA probe, prepared from a 3.4-kilobase segment of the EHEC plasmid, to detect EHEC. The probe hybridized with 106 (99%) of 107 O157:H7 and 34 (77%) of 44 O26:H11, VT-positive strains from patients with colitis, HUS, and diarrheal disease and hybridized with 21 (81%) of 26 VT-positive E. coli of serotypes other than O157:H7 or O26:H11 from patients with hemorrhagic colitis and HUS. We examined 601 other strains, including 18 serotype O26 isolates of H types other than H11, 306 enteropathogenic E. coli, 60 enteroinvasive E. coli, 119 enterotoxigenic E. coli, and 20 isolates from the urinary tract and 77 isolates from the normal intestinal flora; only one (O127:H-) was positive (specificity, 99.8%). Serotype O26:H11, previously considered a classic enteropathogenic E. coli serotype, is now shown to be EHEC.
肠出血性大肠杆菌（EHEC）可引发出血性结肠炎和溶血尿毒综合征（HUS），能产生强效细胞毒素（维罗毒素[VT]或志贺样毒素），并拥有一个质粒（约60兆道尔顿），该质粒编码一种新的菌毛抗原并促进其与上皮细胞的黏附。我们评估了一种由EHEC质粒的3.4千碱基片段制备的DNA探针用于检测EHEC的效果。该探针与107株O157:H7中的106株（99%）以及44株O26:H11中的34株（77%）杂交，这些菌株均来自患有结肠炎、HUS和腹泻病的VT阳性患者，并且与26株来自患有出血性结肠炎和HUS的非O157:H7或O26:H11血清型的VT阳性大肠杆菌中的21株（81%）杂交。我们检测了601株其他菌株，包括18株H11以外H型的O26血清型分离株、306株肠致病性大肠杆菌、60株肠侵袭性大肠杆菌、119株产肠毒素大肠杆菌以及20株来自泌尿道的分离株和77株来自正常肠道菌群的分离株；只有一株（O127:H-）呈阳性（特异性为99.8%）。血清型O26:H11，以前被认为是一种典型的肠致病性大肠杆菌血清型，现在被证明是EHEC。
