Zacharová G, Kubínová L
Institute of Physiology, Czech Academy of Sciences, Prague.
J Muscle Res Cell Motil. 1995 Jun;16(3):295-302. doi: 10.1007/BF00121138.
Stereological methods, using the principle of point counting and unbiased counting frames, for the estimation of muscle area, total fibre area, number of muscle fibres and mean fibre area are described in detail. Their practical application is demonstrated on cross-sections of the rat soleus muscles. It is shown that the efficiency of these methods is high and their results are comparable with those achieved by the conventional manual and image analysis methods. The main advantages of two-dimensional stereological methods in muscle morphometry are pointed out: measurements are made directly on specimens under the microscope and in the simplest implementation do not require sophisticated and expensive technical equipment. Furthermore, unbiased results are obtained, no segmentation and edge effect problems arise and the quantity of work invested in stereological estimation is reasonable. Based upon the study of the efficiency of used stereological methods, a suitable test system for muscle morphometry is proposed.
详细描述了运用点计数原理和无偏计数框的体视学方法，用于估计肌肉面积、总纤维面积、肌纤维数量和平均纤维面积。在大鼠比目鱼肌横截面上展示了这些方法的实际应用。结果表明，这些方法效率高，其结果与传统手工和图像分析方法所获得的结果具有可比性。指出了二维体视学方法在肌肉形态测量中的主要优点：测量直接在显微镜下的标本上进行，最简单的实施方式不需要复杂且昂贵的技术设备。此外，可获得无偏结果，不会出现分割和边缘效应问题，并且体视学估计所投入的工作量合理。基于对所使用的体视学方法效率的研究，提出了一种适用于肌肉形态测量的测试系统。
