Lacy M, Jones J, Whittemore S R, Haviland D L, Wetsel R A, Barnum S R
Department of Microbiology, University of Alabama at Birmingham 35294, USA.
J Neuroimmunol. 1995 Aug;61(1):71-8. doi: 10.1016/0165-5728(95)00075-d.
The expression of chemotactic receptors in the central nervous system is largely unexplored. In this study, we examined human astrocytes and microglia as well as the conditionally immortalized human astrocyte cell line HSC2 for expression of the C5a-anaphylatoxin receptor (C5aR), the interleukin-8 receptor (IL-8R) and the f-Met-Leu-Phe receptor (FMLPR). Using flow cytometry, indirect immunofluorescence and RT-PCR analysis, we demonstrated that astrocytes, microglia and HSC2 cells contain specific RNA and express surface protein for all three chemotactic receptors. These are the first studies to demonstrate definitively the expression of these chemotactic receptors astrocytes and microglia, thereby expanding the types of cells known to express chemotactic receptors. Moreover, these data suggest that these chemotactic receptors may play an important role in mediating the inflammatory response and perhaps other yet undescribed biological phenomena in the central nervous system.
趋化因子受体在中枢神经系统中的表达情况在很大程度上尚未得到研究。在本研究中，我们检测了人类星形胶质细胞、小胶质细胞以及条件永生化人类星形胶质细胞系HSC2中C5a过敏毒素受体（C5aR）、白细胞介素8受体（IL-8R）和N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸受体（FMLPR）的表达。通过流式细胞术、间接免疫荧光和逆转录聚合酶链反应分析，我们证明星形胶质细胞、小胶质细胞和HSC2细胞含有所有三种趋化因子受体的特异性RNA并表达表面蛋白。这些是首次明确证明星形胶质细胞和小胶质细胞表达这些趋化因子受体的研究，从而扩展了已知表达趋化因子受体的细胞类型。此外，这些数据表明这些趋化因子受体可能在介导中枢神经系统的炎症反应以及或许其他尚未描述的生物学现象中发挥重要作用。
