[Percentage of plasma protein-bound and free 17-beta-estradiol, progesterone and testosterone during the sexual cycle and during pregnancy in cows].

Examined were 12 cows with normal sexual cycle and other 12 cows in the last 20 days of pregnancy, all animals belonging to the Black-Pied breed. It was found that plasma protein bound 17-beta estradiol, progesteron, and testosteron during the sexual cycle were 94.726 +/- 0.076, 94-500 +/- 0.125 and 94.122 +/- 0.066 per cent, respectively. In the last twenty days of pregnancy these values rose - 96.911 +/- 0.058, 96.124 +/- 0.074, and 96.040 +/- 0.115, respectively. In testing the binding capacity of the bovine serum albumin it was found that it fixed almost 100 per cent the three ovarial hormones, while the bovine alpha, beta- and gamma-globulin's capacity in this respect was expressed in a negligible percent.