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[临近分娩日的高产奶牛血浆蛋白组分的微量琼脂凝胶电泳定量测定]

[Quantitative determination of blood-plasma protein fractions using micro-agar gel electrophoresis in highly pregnant dairy cows close to the day of delivery].

作者信息

Blauärmel H, Krüger I

出版信息

Arch Exp Veterinarmed. 1976;30(6):925-32.

PMID:66043
Abstract

Ten clinically intact Jersey dairy cows in advanced pregnancy were examined and tested over a span of three hours before to seven hours after parturition. Blood was sampled from them in intervals of ten tp 20 minutes, and the blood plasma was checked total protein and the "classical" fractions. The average total protein value of the plasma samples was 6.5 g/100 ml. The albumin and alpha 1-fractions were inversely proportional the psi-globulin concentrations before, during, and after parturition. The albumin values were on a declining trend three hours prior to parturition and recommenced to rise ten minutes prior to parturition. They were relatively constant after parturition. The pri-globulins went up slightly prior to parturition, but that rise remained statistically unsecured. It is assumed that in a certain period of time from before to somewhen after parturition change of the fractions is caused by the corticoids and sexual hormones.

摘要

对10头临床健康、处于妊娠后期的泽西奶牛在分娩前3小时至分娩后7小时期间进行了检查和测试。每隔10至20分钟从它们身上采集血液样本,并检测血浆中的总蛋白及“经典”组分。血浆样本的平均总蛋白值为6.5 g/100 ml。在分娩前、分娩期间和分娩后,白蛋白和α1组分与ψ球蛋白浓度呈反比。白蛋白值在分娩前3小时呈下降趋势,在分娩前10分钟开始回升。分娩后相对稳定。前球蛋白在分娩前略有上升,但这种上升在统计学上无显著意义。据推测,在分娩前至分娩后一段时间内,这些组分的变化是由皮质类固醇和性激素引起的。

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