[Introduction of an additional H-agglutinating test antigen for the differentiation of specific positive reactions in pullorum disease-fowl typhoid in poultry experimentally infected with Salmonella gallinarum-pullorum and Salmonella enteritidis].

Explored were the possibilities to differentiate the specific positive reactions in case of pullorum disease in experimentally infected (Salmonella gallinarum-pullorum and Salmonella enteritidis) birds with the use of a typhoid test antigen and two H test antigens, obtained by the routinely employed method with formalin from Salmonella enteritidis and Salmonella adeoyo. It was demonstrated that the H-agglutinating antibodies persisted in the peripheral blood of the experimentally infected birds for at least 30 days (as long as the test lasted). The H test antigen of S. enteritidis proved unsuitable to detect the H-agglutinating antibodies only as it retained to a large extent the O antigens. By means of the S. adeoyo H test antigen only the H-agglutinating antibodies in the peripheral blood of S. enteritidis-infected birds could be quickly and precisely demonstrated. A combined serologic examination of serum with the thyphoid test antigen and the S. adeoyo test antigen could be carried out to differentiate the specific positive reactions for establishing pullorum disease from the concomitant agglutination reactions observed in cases of experimental salmonellosis in birds caused by S. enteritidis.