Ivanov P
Vet Med Nauki. 1977;14(9):28-32.
An immunoadsorbent was obtained from a lipopolysaccharide of Salmonella gallinarum-pullorum, employing the method of Eskenazy. It was used to adsorb the O agglutinating antibodies against S. gallinarum-pullcorum, S. enteritidis, S. heidelberg in blood sera of birds. Adsorption of sera was carried out at room temperature for 40 min. It was demonstrated that the product was highly specific. It fully and specifically found the O agglutinating antibodies in the sera of birds infected with S. gallinarum-pullorum and S. enteritidis and in the sera of birds coming from a flock with pullorum infection, however, did not bind the O agglutinating antibodies induced by the O antigens 4 and 5 characteristic of Salmonella heidelberg, resp., group B. A following investigation of the sera, using a S. heidelberg test-antigen, specified the sera of birds infected with S. heidelberg only. The application of the immunoadsorbent makes it possible to speed up the adsorption of the O agglutinating antibodies, and thus, for one or several hours within a single working day the differentiation is carried out of B and D Salmonella infections in birds.
采用埃斯凯纳齐方法，从鸡白痢沙门氏菌的脂多糖中获得了一种免疫吸附剂。它被用于吸附禽类血清中针对鸡白痢沙门氏菌、肠炎沙门氏菌、海德堡沙门氏菌的O凝集抗体。血清吸附在室温下进行40分钟。结果表明该产品具有高度特异性。它能完全且特异地检测出感染鸡白痢沙门氏菌和肠炎沙门氏菌的禽类血清以及来自有鸡白痢感染鸡群的禽类血清中的O凝集抗体，然而，它不结合由海德堡沙门氏菌（分别属于B组）的4和5型O抗原诱导产生的O凝集抗体。随后用海德堡沙门氏菌检测抗原对血清进行检测，仅确定了感染海德堡沙门氏菌的禽类血清。免疫吸附剂的应用使得O凝集抗体的吸附得以加速，因此，在一个工作日内的一两个小时内就能对禽类中的B和D组沙门氏菌感染进行鉴别。
