VanRooij J G, Vinke E, De Lange J, Bruijnzeel P L, Bodelier-Bade M M, Noordhoek J, Jongeneelen F J
Department of Toxicology, University of Nijmegen, The Netherlands.
J Appl Toxicol. 1995 May-Jun;15(3):193-200. doi: 10.1002/jat.2550150309.
Urinary 1-OH-pyrene, a metabolite of pyrene, is a sensitive biological marker for dermal absorption of pyrene in man. In order to determine whether this metabolite is a reliable biomarker of cutaneous absorption of other polycyclic aromatic hydrocarbons (PAHs), the blood-perfused pig ear model was used to compare the dermal absorption flux of pyrene with nine other PAHs after coal tar application. Cumulative absorption of PAHs into the perfusion blood, 200 min after application of an overdose of coal tar, ranged between 830 pmol cm-2 for phenanthrene to less than 4 pmol cm-2 for benzo[b]fluoroanthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[ah]anthracene and indeno[123-cd]pyrene. The results of this study show that when pyrene is used as a marker compound for PAH absorption through pig skin, the cumulative absorption of PAHs with a lower molecular weight will be underestimated: fluorene, tenfold; phenanthrene, 12-fold; anthracene and fluoranthene, ca. twofold. The percutaneous absorption of PAHs with a higher molecular weight than pyrene will be overestimated: e.g. benzo[a]pyrene, sevenfold; indeno [123-cd]pyrene, ca. 100-fold. It is likely that this conclusion is also valid for dermal PAH absorption in man.
尿中1-羟基芘是芘的一种代谢产物，是人体皮肤吸收芘的一种敏感生物标志物。为了确定这种代谢产物是否是其他多环芳烃（PAHs）皮肤吸收的可靠生物标志物，采用血液灌注猪耳模型，比较了煤焦油涂抹后芘与其他9种多环芳烃的皮肤吸收通量。在涂抹过量煤焦油200分钟后，多环芳烃在灌注血液中的累积吸收量，菲为830皮摩尔/平方厘米，而苯并[b]荧蒽、苯并[k]荧蒽、苯并[a]芘、二苯并[ah]蒽和茚并[123-cd]芘则小于4皮摩尔/平方厘米。本研究结果表明，当用芘作为多环芳烃经猪皮肤吸收的标记化合物时，分子量较低的多环芳烃的累积吸收量会被低估：芴，低估10倍；菲，低估12倍；蒽和荧蒽，约低估2倍。分子量比芘高的多环芳烃的经皮吸收量会被高估：例如苯并[a]芘，高估7倍；茚并[123-cd]芘，约高估100倍。这一结论可能对人体皮肤多环芳烃吸收也有效。
